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. 2021 Jan 8;8:591084. doi: 10.3389/fbioe.2020.591084

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Copyright © 2021 Juhl, Merife, Zhang, Lemmon and Donahue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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β-catenin localization and total β-catenin concentration over 96 h and transcriptional activity at 48 and 72 h in hFOB 1.19 cells cultured on either TCPS or pro-osteogenic substrate. Quantification of normalized β-catenin concentration in either the (A) membrane bound cell fraction, (B) nuclear cell fraction, or (D) total β-catenin concentration. (C) Nuclear β-catenin activity quantified by TOPFLASH activity on either TCPS or pro-osteogenic substrate after 48 h and 72 h. n = 4–6 samples with each sample consisting of two replicates. *p < 0.05.