Figure 4.

Inhibition of β-catenin binding to TCF/LEF in hFOB 1.19 cells on TCPS or Pro-Osteogenic Substrates. Evaluation of effect of β-catenin inhibitor, PNU-74564, on (A) Axin2 gene expression and (B) AP Activity in cells cultured in media with inhibitor (+) or without inhibitor (–) on either TCPS or Pro-Osteogenic substrates. *p < 0.05, ^#p < 0.05 compared to all other groups at same time point. n = 5–6 samples with each sample being the average of 3 replicates or 2 replicates for gene expression and AP activity, respectively.