Figure 3.

Proband-Derived iPSCs Recapitulate the Clinical Phenotype of Dyserythropoiesis

(A) Schematic of iPSC differentiation into hematopoietic progenitors followed by ex vivo erythropoiesis.

(B) Flow cytometry analysis of the CD43⁺/CD41⁺/GPA⁺ hematopoietic progenitors harvested on day 10 from control iPSCs.

(C) Flow cytometry analysis of control iPSC-derived erythroblasts by GPA, α₄ integrin, and Band 3.

(D) Flow cytometry analysis of erythroblasts produced in ex vivo erythropoiesis cultures from control- and p1-derived iPSCs.

(E) Wright-stained cytospins from day 10+8 cultures (arrows indicate binucleated erythroblasts). Scale bar 10 μm.

(F) Average growth curves for iPSC lines used in the ex vivo erythropoiesis phase of the culture (mean ± SEM, n = 4 for control and p1; n = 2 for cultures initiated from CDA-I iPSCs).