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. 2021 Jan 8;8:610723. doi: 10.3389/fbioe.2020.610723

TABLE 2.

Purple non-sulfur bacteria as cell factories: the production of heterologous products and overexpression of native products via genetic modification and the production titers thereof.

Organism Product Method Titer References
Rhodobacter Sphaeroides Rs265 Amorphadiene Expression of ads and MVA pathway (mvaA, idi, hcs, mvk, pmk, mvd) (Huembelin et al., 2014) 46.1 mg/L Orsi et al., 2020a
Coenzyme Q10 Overexpression of crtE and ppsR Deletion of crtB, crtC, crtD and crtI 73.2 mg/L Zhu et al., 2017
Lycopene Insertion of crtI4 and dxs Deletion of crtI3, crtC, and zwf 66.0 mg/L Su et al., 2018
Sesquiterpene Insertion of MVA pathway (mvaA, idi, hcs, mvk, pmk, mvd) (Huembelin et al., 2014) Deletion of dxr 7.0 mg/L Orsi et al., 2020b
Valencene Insertion of CnVS and MVA pathway (mvaA, idi, hcs, mvk, pmk, mvd) (Huembelin et al., 2014) 0.4 g/L Beekwilder et al., 2014
Rhodobacter sphaeroides HY01 Hydrogen Overexpression of atp (F1 operon) 8.5 LH2/L Zhang et al., 2016a
Rhodobacter sphaeroides HJ Poly(3-hydroxybutyrate) Insertion of phaA3, phaB2, and phaC1 Deletion of phaZ 1.9 g/L Kobayashi and Kondo, 2019
Rhodobacter capsulatus SB1003 Botryococcene Insertion of dxs, idi, fps 0.1 g/L Khan et al., 2015
Hydrogen Deletion of mmsa 4.7 LH2/L Zhang et al., 2016b
Patchoulol Insertion of P, ispA, dxs and idi, and MVA pathway (mvaA, idi, hcs, mvk, pmk, mvd) (Huembelin et al., 2014) 24 mg/L Troost et al., 2019
Valencene Insertion of CnVS, ispA, and MVA pathway (mvaA, idi, hcs, mvk, pmk, mvd) (Huembelin et al., 2014) 18 mg/L Troost et al., 2019
Rhodopseudomonas palustris TIE-1 Squalene Expression of dxs and a fused crtE-hpnD Deletion of shc 15.8 mg/g DCW Xu et al., 2016
Rhodopseudomonas palustris CGMCC 1.2180 Methane Mutation of nifD at V75A and H201Q 144 nmol/mg total protein Ma et al., 2020
Rhodopseudomonas palustris CGA009 Butanol Insertion of adhE2 0.1 g/L Doud et al., 2017
Hydrogen Expression of nifA Deletion of draT2 1.3 LH2/L Wu et al., 2016
Rhodopseudomonas palustris CEA1001 Canthaxanthin Insertion of crtY, and crtW Deletion of crtC and crtD 0.8 mg/L Giraud et al., 2018
β-carotene Insertion of crtY, and crtW Deletion of crtC and crtD 4.7 mg/L Giraud et al., 2018
Lycopene Deletion of crtC and crtD 7.2 mg/L Giraud et al., 2018
Rhodospirillum rubrum S1 Lycopene Deletion of crtC and crtD 15 mg/L Wang et al., 2012