Figure 4.

EMR2 ligation triggered the activation signal for NLRP3 inflammasome activation and induced ASC oligomerization in THP-1 cells. (A) ELISA analysis of IL-1β in the supernatants of THP-1 cells cultured on plates coated without or with 2A1 mAb in the absence or presence of LPS for 24 h. Cells were either treated without (lane 1) or with additional stimuli (lane 2, 5 mM ATP; lane 3, 100 mg/ml MSU), or 10 μg/ml mouse IgG₁ (lane 4) as a negative control (n=3, mean ± SEM; one-way ANOVA. *p<0.05, ^*** p<0.001). (B) THP-1 cells were cultured on plates coated without or with 2A1 mAb in the absence or presence of LPS for 24 h. The ASC oligomers and relevant components of NLRP3 inflammasome in the cell lysate and pellets were analyzed by Western blotting as indicated.