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. 2020 Feb 17;117(2):561–575. doi: 10.1093/cvr/cvaa040

Figure 7.

Figure 7

ADK expression and DNA methylation in human femoral arterial neointima. (A) Representative IHC staining of ADK on sections of human femoral artery specimens with in-stent restenosis. The left image shows the tunica intima area and the right one shows the tunica media area. ADK-positive cells (brown spots) were shown with DAB staining. (B) Quantification of human stenotic artery specimen in (A) via Image Pro Plus (n = 6 per group). (C and D) Representative IF staining (C) and quantification (D) of 5-mC (red) in the tunica intima area and tunica media area of human stenotic femoral artery (n = 6 per group). (E and F) Representative IHC staining (E) and quantification (F) of KLF4 in the tunica intima area and tunica media area of human stenotic femoral artery (n = 6 per group). For all bar graphs, data are expressed as the means ± SEM, **P < 0.01 (unpaired, two-tailed Student’s t-test).