Figure 3.

Glomeruloid bodies were present in the forebrain, hindbrain, and spinal cord of E14 Tfpi^−/−brain. (A) Coronal sections of anterior forebrain, posterior forebrain, and hindbrain stained for laminin revealed glomeruloid bodies throughout the brain parenchyma. In the sections shown, the anterior forebrain section was at the beginning of the lateral ganglionic eminence. The posterior forebrain section was near its junction with the midbrain as indicated by the presence of the aqueduct of Sylvius, lateral ventricle, choroid plexus, and third ventricle. The hindbrain section was at the level of the aqueduct of Sylvius and fourth ventricle. (B) Magnification of the boxed areas in panel A. Anterior forebrain inset: glomeruloid bodies were present at the bottom of the brain entering from the pial vasculature and in the subventricular zone adjacent to the lateral ventricle (arrows) (scale bar, 60 µm). Posterior forebrain inset: glomeruloid bodies were present throughout the brain parenchyma and adjacent to areas of cell death (dotted black line). Large glomerular bodies originate from pial vessels adjacent to the lateral ventricle (arrow) (scale bar, 80 µm). Hindbrain inset: glomeruloid bodies were present throughout the parenchyma, and pial vessels at the pia-brain interface were disorganized (arrows) (scale bar, 100 µm). Glomeruloid bodies were present in the spinal cord (SC). Adjacent to the spinal cord is the vertebral body primordium (VBP) (scale bar, 100 µm). Corresponding coronal sections from a Tfpi^+/+ littermate are presented in supplemental Figure 2.