Figure 5.
Plg-RKT −/− platelets retain reduced membrane-bound plasminogen. Washed platelets (2 × 108 platelets/mL) in modified Tyrodes buffer (pH 7.45) from Plg-RKT+/+ (hatched bars) or Plg-RKT−/− mice (solid bars) were left unstimulated or stimulated in the presence of 2 mM CaCl2 with 100 ng/mL CVX + 100 nM thrombin. Annexin V-AF633 with 2 mM CaCl2 was added after 45 minutes. Platelet-derived plasminogen was detected using anti-plasminogen DL488 antibody. A minimum of 10 000 events was collected using a Fortessa flow cytometer. *P < .05 compared with Plg-RKT+/+ stimulated platelets. Data are expressed as mean ± SEM, n ≥ 4.