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Overview of the identified cross-linked peptides. (A) Detected XLs are indicated in red (inter-links) or blue (intra-links). A total number of 126 XLs with an estimated average of 8 links per interface were identified. (B) The generated pairwise docking models were filtered out by XL peptides and were structurally aligned together to represent the C5b–C9 complex initiating the pore by multiple C9 components. This model is supported by 38 inter-XLs.
