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. 2021 Jan 8;11:612620. doi: 10.3389/fphar.2020.612620

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Copyright © 2021 Jin, Jin, Wu, Liu, Zhu, Shou and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of HSF on PD-1, TIGIT, and CTLA-4 in the blood and lung samples of the breast cancer postoperative metastasis mice. (A) Representative image of PD-1, TIGIT, and CTLA-4 population in the blood. (B) Flow cytometry of PD-1, TIGIT, and CTLA-4, expressed as percentage of CD8⁺ T cells in the blood. (C) Representative image of PD-1, TIGIT, and CTLA-4 population in the lungs. (D) Flow cytometry of PD-1, TIGIT, and CTLA-4, expressed as percentage of tumour-infiltrating CD8⁺ T cells in the lungs. Values are means ± SEM; n = 10 mice/group; *p < 0.05, **p < 0.01, ***p < 0.001 (unpaired t-tests).