Subject	Biology
Specific subject area	Molecular Biology, Cell Biology, Human Pluripotent Stem Cells, Lens Epithelial Cells, Single Cell Transcriptomics
Type of data	Table Figure
How data were acquired	Stem cell culture and differentiation. RNA-Seq (10X Genomics single cell 3′ mRNA-prep kit and Illumina NextSeq 500). Transcriptomic data analyses (Seurat guided clustering suite version 3; Bioconductor R package; DAVID gene ontology).
Data format	Raw fastq files
Parameters for data collection	Human pluripotent stem cells were differentiated to lens epithelial cells using established methods (Murphy et. al, 2018). ROR1e LECs were harvested on day 16 and expanded for a further 7 days in optimised medium (Murphy et. al, 2018). Cells were collected 7 days after harvest and immediately processed for scRNA-seq.
Description of data collection	Total RNA was isolated from ROR1e LECs. cDNA library was constructed and scRNA-seq performed. Bioinformatics performed using Seurat guided clustering suite version 3.
Data source location	School of Medicine, Western Sydney University, Campbelltown, Australia
Data accessibility	ArrayExpress accession number E-MTAB-9178 https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-9178/
Related research article	Umala Dewi et al, 2020, A simplified method for production of human lens epithelial cells and light-focusing micro-lenses from pluripotent stem cells, Exp. Eye Res. Experimental Eye Research. doi.org/10.1016/j.exer.2020.108317.