Table 1.
Purification summary of Peroxidase from Moringa oleifera leave.
| Volume (ml) | Activity (μmol/min/ml) | Protein Concentration (mg/ml) | Specific Activity (μmol/min/mg) | Total Activity (μmol/min) | Total Protein (mg) | Purification Fold | Recovery | |
|---|---|---|---|---|---|---|---|---|
| Crude | 86 | 14.0695 ±1.0095 |
3.495 ±0.186 |
4.0256 ±0.125 |
1,209.9 | 300.57 | 1 | 100 |
| Aqueous Two-Phase Partitioning System | 18 | 176.88 ±25.95 |
4.589 ±0.3743 |
38.544 ±0.288 |
3,183.8 | 82.60 | 9.57 | 263.13 |
| Gel Filtration on Sephadex G-100 | 288 | 3.5342 ±0.3550 |
0.250 ±0.05 |
14.1368 ±0.30 |
1,017.8 | 72.00 | 3.51 | 84.12 |