Figure 5.

The effects of cladosporin treatment on HCC cell lines. (A) Huh7 and SNU398 cells were treated with different concentrations of cladosporin for 3 days. Cell viability was measured by MTT assay (n = 12). (B) Cladosporin affects the number of single cell‐derived colonies as assayed 2 weeks following seeding (n = 6‐8). (C) Protein expression levels of cleaved‐Caspase 3 in Huh7 and SNU398 cells, and the intensity was quantified relative to β‐actin (n = 5). Huh7 and SNU398 were treated with cladosporin for 3 days. (D) Representative images of migrating cells with cladosporin and (E) quantification of the number of migrating cells (n = 20‐25). Quantification of all data were relative to the negative control group. Data are presented as mean ± SEM. *P < .05; **P < .01; ***P < .001, by the Mann‐Whitney test. HCC, hepatocellular carcinoma; FC, fold change