Fig. 7.
Repetitive priming under diurnal freezing (DF) induced similar effects on transcription but distinct chromatin responses in Brachypodium distachyon. (a) Relative transcript levels during a first (P1) and second (P2) episode of priming under DF separated by a 3‐d recovery from DF (R) in COR genes CBFs, IRI, COR410–413 and VRN1. (b) Relative levels of epigenetic marks H3, H3K27me3 (k27), H3K4me2 (4me2) and H3K4me3 (4me3) in CTR, P1, R and P2. (c) Relative global levels of DNA methylation in CTR, P1, R and P2. (d) Summary of the epigenetic change on COR genes and global DNA methylation levels during repeated priming. Different letters indicate statistical difference; P < 0.05; error bars represent SD between three biological replicates.