Fig. 4.

WRKY21 positively affects phosphate (Pi) accumulation in rice (Oryza sativa). (a) Phenotype of WRKY21 transgenic plants and wild‐type plants grown under HP (200 μM Pi) and LP (10 μM Pi) hydroponic conditions. Bars, 10 cm. (b) Pi concentration analysis in leaf tissues and roots of lines (as above). Four‐leaf‐old seedlings were grown under full‐strength Yoshida nutrient solution supplied with HP (200 μM Pi) or LP (1 μM Pi) until the seventh leaf blades were fully expanded. Pi concentration was measured in plants grown under HP (left) and LP (right) conditions. Leaf blades, leaf sheaths and roots were collected for measurement. Error bars indicate ± SD (n = 4). Data significantly different from the corresponding controls are indicated (**, P < 0.01; Student’s t‐test). FW, fresh weight.