| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Gamborg B-5 basal medium | Phyto Technology | Cat#G398 |
| Sucrose | ABCONE | Cat#57501 |
| 2-(N-morpholino) ethanesulfonic acid (MES) | BBI Life Sciences | Cat#145224948 |
| Phytagel | Sigma-Aldrich | Cat#7101052-1 |
| Agar Bacteriological Grade | Shanghai Jiafeng | Cat#H8145 |
| Triton X-100 | Sigma-Aldrich | Cat#9002931 |
| KCl | Sigma-Aldrich | Cat#7447407 |
| NaCl | Sigma-Aldrich | Cat#7647145 |
| MgCl2·6H2O | Sigma-Aldrich | Cat#7791186 |
| Tris base | Sigma-Aldrich | Cat#77861 |
| DAPI | AAT Bioquest | Cat#28718903 |
| 2-Mercaptoethanol | Ruibio | Cat#60242 |
| Spermine | Sigma-Aldrich | Cat#85590 |
| Eva green dye | Biotium | Cat#31000 |
| Phusion high-fidelity DNA polymerase | Thermo Fisher Scientific | Cat#022021 |
| Complete protease inhibitor cocktail | Merck | Cat#04693132001 |
| Critical commercial assays | ||
| MinElute Reaction Cleanup Kit | QIAGEN | Cat#28204 |
| TruePrep DNA Library Prep Kit v2 | Vazyme Biotech | Cat#TD50102 |
| TruePrep Index Kit v2 | Vazyme Biotech | Cat#TD202 |
| 2× NEBNext high-fidelity PCR mix | New England Biolabs | Cat#M0541L |
| AMPure beads | Beckman | Cat#A63880 |
| Deposited data | ||
| ATAC-seq, ChIP-seq, and RNA-seq experiment data | This paper | BioProject PRJCA002620, Beijing Institute of Genomics Data Center (http://bigd.big.ac.cn) |
| Experimental models: organisms/strains | ||
| A. thaliana: Col-0 | N/A | N/A |
| Software and algorithms | ||
| R version 3.6 | The R Foundation | RRID: SCR_001905 https://www.r-project.orgl |
| Adobe Photoshop CC 2018 | Adobe Acrobat | N/A |
| Adobe Illustrator CC 2018 | Adobe Acrobat | N/A |
| Fastp | (Chen et al., 2018) | RRID: SCR_016962 https://github.com/OpenGene/fastp |
| FastQC v0.11.7 | FastQC | RRID:SCR_014583 http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ |
| MulitQC v1.6 | (Ewels et al., 2016) | https://multiqc.info/ |
| Bowtie2 v2.3.4.3 | (Langmead and Salzberg, 2012) | http://bowtie-bio.sourceforge.net/bowtie2/ |
| Samtools v1.9 | (Li et al., 2009) | RRID: SCR_002105 http://www.htslib.org/ |
| sambamba v0.6.7 | (Tarasov et al., 2015) | https://lomereiter.github.io/sambamba/ |
| bedtools v2.25.0 | (Quinlan and Hall, 2010) | RRID: SCR_006646 https://bedtools.readthedocs.io/en/latest/ |
| deepTools v3.1.2 | (Ramirez et al., 2014) | RRID: SCR_016366 https://deeptools.readthedocs.io/en/develop/ |
| featureCounts v1.6.2 | (Liao et al., 2014) | RRID: SCR_012919 http://bioinf.wehi.edu.au/featureCounts/ |
| DESeq2 v1.26.0 | (Love et al., 2014) | RRID: SCR_015687 https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| MACS2 v2.1.2 | (Zhang et al., 2008) | https://github.com/taoliu/MACS |
| DiffBind v2.14.0 | (Ross-Innes et al., 2012) | RRID: SCR_012918 https://bioconductor.org/packages/release/bioc/html/DiffBind.html |
| ChIPseeker v1.22.0 | (Yu et al., 2015) | https://bioconductor.org/packages/release/bioc/html/ChIPseeker.html |
| HOMER v4.10 | (Heinz et al., 2010) | RRID: SCR_010881 http://homer.ucsd.edu/homer/ |
| Intervene v0.6.1 | (Khan and Mathelier, 2017) | https://intervene.readthedocs.io/en/latest/ |
| Integrative Genomics Viewer | (Robinson et al., 2011) | RRID: SCR_011793 http://software.broadinstitute.org/software/igv/ |
| Other | ||
| Eppendorf realplex2 | Eppendorf | Cat#A248709R |
| The BD FACSAria II | BD | N/A |
| Percival chamber | Percival | N/A |