Skip to main content
. 2021 Jan 21;2(1):100289. doi: 10.1016/j.xpro.2020.100289
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

Gamborg B-5 basal medium Phyto Technology Cat#G398
Sucrose ABCONE Cat#57501
2-(N-morpholino) ethanesulfonic acid (MES) BBI Life Sciences Cat#145224948
Phytagel Sigma-Aldrich Cat#7101052-1
Agar Bacteriological Grade Shanghai Jiafeng Cat#H8145
Triton X-100 Sigma-Aldrich Cat#9002931
KCl Sigma-Aldrich Cat#7447407
NaCl Sigma-Aldrich Cat#7647145
MgCl2·6H2O Sigma-Aldrich Cat#7791186
Tris base Sigma-Aldrich Cat#77861
DAPI AAT Bioquest Cat#28718903
2-Mercaptoethanol Ruibio Cat#60242
Spermine Sigma-Aldrich Cat#85590
Eva green dye Biotium Cat#31000
Phusion high-fidelity DNA polymerase Thermo Fisher Scientific Cat#022021
Complete protease inhibitor cocktail Merck Cat#04693132001

Critical commercial assays

MinElute Reaction Cleanup Kit QIAGEN Cat#28204
TruePrep DNA Library Prep Kit v2 Vazyme Biotech Cat#TD50102
TruePrep Index Kit v2 Vazyme Biotech Cat#TD202
2× NEBNext high-fidelity PCR mix New England Biolabs Cat#M0541L
AMPure beads Beckman Cat#A63880

Deposited data

ATAC-seq, ChIP-seq, and RNA-seq experiment data This paper BioProject PRJCA002620,
Beijing Institute of Genomics Data Center (http://bigd.big.ac.cn)

Experimental models: organisms/strains

A. thaliana: Col-0 N/A N/A

Software and algorithms

R version 3.6 The R Foundation RRID: SCR_001905 https://www.r-project.orgl
Adobe Photoshop CC 2018 Adobe Acrobat N/A
Adobe Illustrator CC 2018 Adobe Acrobat N/A
Fastp (Chen et al., 2018) RRID: SCR_016962 https://github.com/OpenGene/fastp
FastQC v0.11.7 FastQC RRID:SCR_014583 http://www.bioinformatics.babraham.ac.uk/projects/fastqc/
MulitQC v1.6 (Ewels et al., 2016) https://multiqc.info/
Bowtie2 v2.3.4.3 (Langmead and Salzberg, 2012) http://bowtie-bio.sourceforge.net/bowtie2/
Samtools v1.9 (Li et al., 2009) RRID: SCR_002105 http://www.htslib.org/
sambamba v0.6.7 (Tarasov et al., 2015) https://lomereiter.github.io/sambamba/
bedtools v2.25.0 (Quinlan and Hall, 2010) RRID: SCR_006646 https://bedtools.readthedocs.io/en/latest/
deepTools v3.1.2 (Ramirez et al., 2014) RRID: SCR_016366 https://deeptools.readthedocs.io/en/develop/
featureCounts v1.6.2 (Liao et al., 2014) RRID: SCR_012919 http://bioinf.wehi.edu.au/featureCounts/
DESeq2 v1.26.0 (Love et al., 2014) RRID: SCR_015687 https://bioconductor.org/packages/release/bioc/html/DESeq2.html
MACS2 v2.1.2 (Zhang et al., 2008) https://github.com/taoliu/MACS
DiffBind v2.14.0 (Ross-Innes et al., 2012) RRID: SCR_012918 https://bioconductor.org/packages/release/bioc/html/DiffBind.html
ChIPseeker v1.22.0 (Yu et al., 2015) https://bioconductor.org/packages/release/bioc/html/ChIPseeker.html
HOMER v4.10 (Heinz et al., 2010) RRID: SCR_010881
http://homer.ucsd.edu/homer/
Intervene v0.6.1 (Khan and Mathelier, 2017) https://intervene.readthedocs.io/en/latest/
Integrative Genomics Viewer (Robinson et al., 2011) RRID: SCR_011793 http://software.broadinstitute.org/software/igv/

Other

Eppendorf realplex2 Eppendorf Cat#A248709R
The BD FACSAria II BD N/A
Percival chamber Percival N/A