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. 2021 Jan 21;2(1):100282. doi: 10.1016/j.xpro.2020.100282

Figure 4.

Figure 4

Software demonstration of cytosolic Ca2+ measurement using ratiometric Fura 2 dye

(A) Representative images of the experiment: channel 1 (340 nm) is presented in the upper left panel, channel 2 (387 nm) is in the upper right panel, the overlay channel 1/channel 2 is in the bottom left panel, and the ratio is in the bottom right panel.

(B) Representative traces (Quantify → Graph tabs) referring to the experiment shown in (A).