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Cytosolic Ca2+ measurement using ratiometric Fura 2 dye
(A) Representative image of basal level of cytosolic Ca2+ (340/387 nm ratio) in SK-MEL-19 melanoma cells at 500 s (just before adding thapsigargin).
(B) Representative image of thapsigargin-stimulated cytosolic Ca2+ increase (340/387 nm ratio) in SK-MEL-19 melanoma cells at 800 s.
(C) Experimental curve showing the effect of thapsigargin added at 660 s on fluorescence ratio (340/387 nm), representing the intracellular Ca2+ level.
