REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Fetal bovine serum	Thermo Fisher Scientific	#Cat1265029
Chemicals, peptides, and recombinant proteins
NaCl	Sigma-Aldrich	#CatS9888; CAS 7647-14-5
KCl	Sigma-Aldrich	#CatP3911; CAS 7447-40-7
Na2SO4	Sigma-Aldrich	#Cat239313; CAS 7757-82-6
Na2HPO4	Sigma-Aldrich	#Cats9763; CAS 7558-79-4
KH2PO4	Sigma-Aldrich	#CatP0662; CAS 7778-77-0
Glucose	Sigma-Aldrich	#CatG7021; CAS 492-62-6
Phenol red	Sigma-Aldrich	#Catp3532; CAS 143-74-8
Trypsin	Thermo Fisher Scientific	#Cat15400054; CAS 9002-07-7
HEPES	Sigma-Aldrich	#CatH4034; CAS 7365-45-9
NaHCO₃	Sigma-Aldrich	#CatS5761; CAS 144-55-8
DMEM high glucose	Sigma-Aldrich	#CatD5648
Penicillin-streptomycin	Thermo Fisher Scientific	#Cat15140122
CaCl2	Sigma-Aldrich	#CatC4901; CAS 10043-52-4
MgCl2·6H2O	Sigma-Aldrich	#CatM9272; CAS 7791-18-6
MgSO4·7H2O	Sigma-Aldrich	#Cat230391; CAS 10034-99-8
Sucrose	Sigma-Aldrich	#CatS5016; CAS 57-50-1
Trypan blue	Sigma-Aldrich	#CatT8154; CAS 72-57-1
Fura 2-AM	Thermo Fisher Scientific	#CatF1201; CAS 108964-32-5
Thapsigargin	Sigma-Aldrich	#CatT9033; CAS 67526-95-8
Ionomycin	Sigma-Aldrich	#CatI0634; CAS 56092-82-1
DMSO	Sigma-Aldrich	#Cat472301; CAS 67-68-5
Poloxamer 407	Sigma-Aldrich	#CatP2443; CAS 9003-11-6
Hoechst 33342	Thermo Fisher Scientific	#Cat62249; CAS 23491-52-3
Experimental models: cell lines
Human: SK-MEL-19 cells	Esteves et al., 2020	https://doi.org/10.1016/j.ceca.2020.102241
Software and algorithms
GraphPad Prism 6.0	Esteves et al., 2020	https://www.graphpad.com/scientific-software/prism/
Materials and equipment
Circulating water bath	PolyScience	#CatAD15H200-A12
Precision scale	Shimadzu	#CatAX200
Magnetic stirrer	IKA	#CatZ403644
pH meter	Quimis Aparelhos Científicos	#CatQ400A5
Purelab Option-Q	ELGA	#CatLC197
Vertical laminar flow cabinet	Esco Technologies	#CatSVE-4A3
Sterifil aseptic system	Sigma-Aldrich	#Cat171
Whatman membrane filters	Sigma-Aldrich	#Cat10401770
Water bath	PolyScience	#CatWDO5A12E
Treated cell culture flask	Nest Scientific	#Cat7080003
MCO-19AIC cell incubator	Panasonic	#CatMCO-19AIC
Sorvall ST16R centrifuge	Thermo Fisher Scientific	#CatST16R
DM IL LED inverted optical microscope	Leica Microsystems	#CatDMIL-LED
Neubauer chamber	New Optik	#Cat1110000
Glass bottom dishes	MatTek	#CatP35G-1.5-14-CGRD
Leica AF6000 fluorescence image system	Leica Microsystems	#CatDMI6000B
CELLview cell culture dish	Greiner Bio-One	#Cat627860