REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Nitric acid	Merck KGaA	Cat#1006301000
Ethanol	Merck KGaA	Cat#1117271000
Paraffin wax	Sigma-Aldrich	Cat#327212
Poly-L-lysine	Sigma-Aldrich	Cat#P4707
Culture medium (DMEM-F12)	Thermo Fisher	Cat#12500062
Fetal bovine serum	Thermo Fisher	Cat#12657029
Polystyrene beads	Polysciences	Cat#17134-15
Immersion oil	Nikon	Cat#MXA22165
CO2 gas	Alta Pressão	N/A
Glutaraldehyde	Electron Microscopy Sciences	Cat#16000
Sodium cacodylate trihydrate	Sigma-Aldrich	Cat#C0250-10G
Sucrose	Sigma-Aldrich	Cat#S7903
Magnesium chloride	Sigma-Aldrich	Cat#M8266
Software and algorithms
ImageJ	NIH	https://imagej.nih.gov/ij/
KaleidaGraph	Synergy Software	https://www.synergy.com/
Other
Coverslips	Knittel Glass	Cat#VD12020Y1A.01
Glass-bottom dishes	MatTek Life Sciences	Cat#P35G-0-10-C
35 mm culture dishes	Corning	Cat#430165
Glass-writing diamond pen	Agar Scientific	Cat#AGT5347
Autoclave	Prismatech	Cat#CS18
Drill and hole saw	Beltec	Cat#LB100
Sharp knife (bistoury)	ABC Instrumentos Cirúrgicos	Cat#0015
Tweezers	Electron Microscopy Sciences	Cat#78510-0
Glass stick	Sigma-Aldrich	Cat#S4522-6EA
Hot plate	Ika	Cat#C-MAG HS 7
Biological safety cabinet	Esco	Cat#AB2-3S1
Cell incubator	Thermo Fisher	Cat#4110
Micropipette automatic	Gilson	Cat#FA10006M
Neubauer chamber	Sigma-Aldrich	Cat#BR717805-1EA
OT laser	IPG Photonics	Cat#YLR-5-1064-LP
Inverted microscope	Nikon	Cat#EclipseTE300
Stage positioning	PI	Cat#P-545.3R8S
CO2 and temperature chamber	Adapted	N/A
Optical table	Thorlabs	Cat#T1020CK
Objective lens	Nikon	Cat#PLAN APO 100X 1.4 NA DIC H
Optical power meter	Newport	Cat#843-R
Microscope camera	Hamamatsu	Cat#C2400
Frame grabber	Scion Corporation	Cat#FG7
Fume hood	HQ	Cat#SP80N
Critical-point dryer	Bal-tech	Cat#CPD 030
Carbon adhesive tape	Electron Microscopy Sciences	Cat#77825-06
Stubs	Agar Scientific	Cat#AG16144
Gold sputter-coater	Bal-tech	Cat#SCD 050
Scanning electron microscope	FEI	Cat#Quanta 250