Fig. 3.

PRE-084 fails to enhance endothelial barrier function in the siRNA-mediated knockdown of σ1. A. The traces show changes in mean TER in the non-targeting RNA (NT) and SIGMAR1 siRNA-mediated knockdown (σ1 kd) groups, before after treatment with 100 μM PRE-084 or vehicle at time t=0 h. B. Time-dependent changes in barrier function in response to PRE-084 or vehicle for each group are also shown using normalized TER values. Normalized TER was calculated by dividing values at each time point by the TER value just prior to the addition of PRE-084 or vehicle (t = 0 h). C. Expanded individual and mean TER for each group is shown for the time point at 3 h after addition of vehicle or 100 μM PRE-084. Data were analyzed by two-way ANOVA followed by Sidak’s test. D. Expanded data for the change in TER (%) from t = 0 h for each group at 3 h after the addition of vehicle or PRE-084 is also shown. An unpaired t test was used for analysis. N= 6–8 HUVEC monolayers per group. P values are shown for all comparisons. Some time points in panel B appear not to have an error bar because the value of the SEM to be plotted was smaller than the size of the symbol.