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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Microcirculation. 2020 Apr 26;27(6):e12620. doi: 10.1111/micc.12620

Fig. 4.

Fig. 4.

PRE-084 enhances glycolytic ATP production in HUVEC. Confluent HUVEC monolayers were treated either with 100 μM PRE-084 or vehicle control for 3 h and then subjected to the ATP production rate assays using the Agilent SeaHorse XFp system. The ATP production rate assay determines the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) before and after addition of oligomycin and later the rotenone/antimycin-A cocktail. A. Assay profile obtained from Agilent Technologies. © Agilent Technologies, Inc, Reproduced with Permission, Courtesy of Agilent Technologies, Inc. B. OCR, and C, ECAR of controls and PRE-084-treated cells over time during the protocol. Additional parameters calculated from the ATP production rate assay include the glycolytic ATP (D), mitochondrial ATP (E), and total ATP (F) production rates. For each parameter, the two groups were compared using an unpaired t-test. N=9 HUVEC monolayers for each group. P values are shown for all comparisons.