Figure S2.

Library composition and number of replicates affect screening results. Distribution of phenotypic scores of all sgRNA groups in four different analysis modes. Phenotypic score of a sgRNA group was calculated based on the average phenotypic scores as follows. Top left: A single sgRNA from a single replicate. Top right: A single sgRNA averaged between two replicates. Bottom left: Two sgRNAs from a single replicate. Bottom right: Two sgRNAs averaged between two replicates.