Figure 2.

ADV inhibited proliferation of stimulated T cells and pre-activated T cells. (A–H) Resting human T cells freshly stimulated (black ) or pre-activated (grey) with anti-CD3/CD28 antibodies were cultured with increasing concentrations of ADV (A–D) or Cladribine (E–H) for 72 (A, E) or 96 h (B–D, F–H). (A, E) DNA-synthesis was determined by standard [³H]-thymidine uptake. [³H]-TdR incorporation is shown as mean percentage ± SEM of DNA synthesis in relation to control cultures (vehicle) set to 100%. (B, F) CFSE dilution of stimulated T cells and pre-activated T cells determined as the percentage of dividing cells. Data are presented as the mean ± SEM of n = 3 independent experiments. Histograms of CFSE staining in freshly stimulated T cells (C, G) and pre-activated T cells (D, H) are shown from a representative experiment.