Figure 5.

ADV induced phosphorylation of p53 in freshly stimulated and pre-activated T cells. Resting human T cells were freshly stimulated (A–C) or pre-activated for 48 h (D–F) with anti-CD3/CD28 antibodies and cultured with increasing concentrations of ADV. After additional 24 h incubation, cells were lysed and analyzed by Western blot. (A, C) Representative Western blot images of p-Ser15-p53 and total p53, β-actin was used as a loading control. Relative expression of p-Ser15-p53 (B, E) and p53 (C, F) is shown based on densitometric quantification. Quantitative data are presented as the mean ± SEM from n = 3 independent experiments. Statistical analysis was performed with One-Way ANOVA and Dunnett’s Multiple Comparison Analysis Test as post hoc test. (****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05).