Figure 7.

ADV induced cell death in freshly stimulated and pre-activated T cells. Resting human T cells were freshly stimulated (A, C) or pre-activated for 48 h (B, D) with anti-CD3/CD28 antibodies and cultured with increasing concentrations of ADV. Cells were stained after 72 h with Annexin V-FITC/propidium iodide (PI) for flow cytometric analysis. (A, B) Representative flow cytometry dot plots, and (C, D) quantification of early and late apoptotic cells of n = 3 independent experiments are shown. Statistical analysis was performed with One-Way ANOVA and Dunnett’s Multiple Comparison Analysis Test as post hoc test. (****P ≤ 0.0001, **P ≤ 0.01, *P ≤ 0.05).