Figure 9.

ADV induced up-regulation of PUMA in stimulated T cells. Resting human T cells were freshly stimulated (A, B) or pre-activated for 48 h (C, D) with anti-CD3/CD28 antibodies and cultured with increasing concentrations of ADV. Cells were lysed after 24 h, respectively, and analyzed by Western blot. (A, C) Representative Western blot images of PUMA expression, β-actin was used as a loading control. (B, D) Relative expression of PUMA based on densitometric quantification. Quantitative data are presented as the mean ± SEM from n = 6 (B), n = 3 (D) independent experiments. Statistical analysis was performed with One-Way ANOVA and Dunnett’s Multiple Comparison Analysis Test as post hoc test. (***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05).