Fig. 2.

Appearance of rice oscuc3 knockout mutants. (a) Schematic diagram of OsCUC3 gene structure and the CRISPR/Cas9 target sites. The black triangles indicate the two individual target sites for knockout of OsCUC3. The PAM site is underlined. The mutation site is shown in red. (b, c) The whole plant phenotype of oscuc3‐KO1 and oscuc3‐KO2. (d–g) Cross‐section of the leaf sheath of oscuc3‐KO1, oscuc3‐KO2 and their non‐KO control. (h, i) Fusion of leaf blade in oscuc3‐KO1 and oscuc3‐KO2. The fusion region outlined by the small white rectangle is shown in detail in the bottom right corner. The arrows indicate the fusion of the leaf blades. (j–m) Knocking out OsCUC3 leads to fusion in filaments. The arrow indicates the fusion of two filaments of oscuc3‐KO2. Bars, 500 μm.