Fig. 4.
OsCUC1 and OsCUC3 dimerize and function redundantly in rice. (a, b) BiFC assays showing the heterodimerization and homodimerization of OsCUC1 and OsCUC3. Co‐expression of GFPC‐OsCUC3 plus GFPN, GFPC plus GFPN‐OsCUC3, GFPC‐OsCUC1 plus GFPN, and GFPC plus GFPN‐OsCUC1 were used as negative controls. Bars, 5 μm. (c) Interaction between OsCUC1 and OsCUC3, analysed by in vitro pull‐down assay. Recombinant GST‐OsCUC1 and MBP‐OsCUC3 proteins were used for the pull‐down assay. IB, immunoblot. (d) Interaction between OsCUC1 and OsCUC3, analysed by in vivo CoIP assay. After the co‐transformation of OsCUC1‐MYC and OsCUC3‐GFP in rice protoplasts, total proteins of protoplasts were immunoprecipitated using an anti‐MYC antibody and were detected with anti‐GFP and anti‐MYC antibodies. IB, Immunoblot; IP, immunoprecipitation. (e) The phenotypes of +/+ oscuc3/oscuc3, oscuc1/+ oscuc3/oscuc3 and oscuc1/oscuc1 oscuc3/oscuc3. (f) The fusion of leaf sheath and twisted‐rolling leaf in oscuc1/+ oscuc3/oscuc3. The fusion region of the leaf sheath outlined by a small white rectangle in the main image is shown in detail in the top right corner; the arrow indicates the twisted‐rolling leaf. Bar, 500 μm. (g) The enhanced defects in the oscuc1/oscuc1 oscuc3/oscuc3 double mutant. Bar, 500 μm.