FIGURE 2.

Bioinformatic analysis predicts P. tunicata EAR28894 hypothetical protein as an S‐layer protein. (a) Structure prediction by I‐TASSER. The top five threading templates are listed on the right, and the structural model for top‐ranked template is shown on the left. EAR28894 threaded onto the structure of Caulobacter RsaA (PDB ID 5n8p) as shown in the top left inset box with the template colored in gray. (b) Genomic context surrounding the EAR28894 gene in P. tunicata D2 and related genes in other genomes. Immediately upstream of the EAR28894 gene is a type II secretion gene cluster, suggesting a possible dedicated operon for type II S‐layer secretion