Skip to main content
. 2020 Sep 15;114(6):979–990. doi: 10.1111/mmi.14588

FIGURE 3.

FIGURE 3

TEM imaging of purified EAR28894 reveals a paracrystalline lattice with square (p4) symmetry. (a) Purification of EAR28894 by ultracentrifugation (L1) followed by detergent washes (L2). L1 samples predominantly contain the ~57 kDa band (EAR28894) but also contained other impurities. L1 was, therefore, further purified using RIPA detergent washes to L2, which produced a single band on the gel. (b) TEM imaging reveals a paracrystalline lattice with a square (p4) symmetry. There are approx. 11 subunits per 100 nm, suggesting an inter‐unit spacing of approx. 9.1 nm. (c) Quantitative estimate of inter‐unit spacing based on averages of N = 5 independent measurements from TEM images. Scale bars = 100 nm