Table 3.

Specific enzymatic activity of DHFR (mU/mg) measured in the untreated cells and upon treatment with the selected gold(I) compounds at the concentration causing the maximum inhibition: 6 μM for both A17 and SKBR3, 8 μM for MDA-MB231.

	Time (hours)	A17	MDA-MB231	SKBR3
Cells without treatment (control)	4	1.17 ± 0.06	5.38 ± 0.38	0.72 ± 0.05
	12	4.77 ± 0.78	0.64 ± 0.02	0.67 ± 0.10
Cells treated with compound 5	4	1.07 ± 0.02	2.37 ±0.39	0.54 ± 0.04
	12	1.66 ± 0.07	0.42 ± 0.01	0.29 ± 0.05
Cells treated with compound 6	4	1.03 ± 0.03	1.75 ± 0.34	0.64 ± 0.06
	12	2.01 ± 0.14	0.55 ± 0.002	0.34 ± 0.01