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. Author manuscript; available in PMC: 2021 Jan 22.
Published in final edited form as: J Vis Exp. 2019 Aug 28;(150):10.3791/60230. doi: 10.3791/60230

Figure 1: Establishment of primary mouse astrocyte cultures.

Figure 1:

(A) Steps required for dissection of P2–P4 mouse brains. (B) (i,iv) Brightfield images of mixed glial cultures treated (i) and non-treated (iv) with AraC. Red arrowheads indicate contaminant microglia. (ii,v) Images of purified astrocyte cultures treated (ii) and non-treated (v) with AraC. Blue arrowheads indicate contaminant oligodendrocytes. (iii,vi) Confocal images of purified astrocyte cultures treated (iii) and non-treated (vi) with AraC. Green shows GFAP staining and DAPI (blue) labels all nuclei. (C) Percentage of GFAP-positive cells. Data represents mean ± SEM. ***p < 0.001, unpaired t-test. Please click here to view a larger version of this figure.