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. Author manuscript; available in PMC: 2021 Jan 22.
Published in final edited form as: J Vis Exp. 2019 Aug 28;(150):10.3791/60230. doi: 10.3791/60230

Figure 2: Quantitative analyses of cargo dynamics in astrocytes.

Figure 2:

(A,E,I) Left panels: Astrocytes expressing EAAT1-GFP (A), Cx43-GFP (E) or treated with a probe that labels late endosomes and lysosomes (I). Red boxes indicate regions used to analyze particle dynamics. Right panels: Original and color-coded kymographs showing trajectories for the indicated cargos. Red lines represent retrograde-moving cargos, green lines anterograde-moving cargos, and blue lines non-motile cargos. (B,F,J) Percentage of stationary and motile particles. (C,G,K) Quantification of anterograde and retrograde velocity and (D,H,L) run length. Data represents mean ± SEM. Please click here to view a larger version of this figure.