Skip to main content
. 2021 Jan 11;6(1):e141341. doi: 10.1172/jci.insight.141341

Figure 4. Extracellular calcium is essential for CPB-induced upregulation of IL-8 and TNF-α.

Figure 4

(A and B) When THP-1 cells were sheared in the calcium-free media or in the presence of EGTA, a calcium chelator, the mRNA upregulation of IL8 (A) and TNFA (B) was markedly blunted compared with the respective control media (n = 3 replicates/group). *P < 0.05, 1-way ANOVA and post hoc Tukey’s test. (C) Protein levels of IL-8 and TNF-α in the THP-1 cells sheared in calcium-free media were significantly lower than those in the control media (n = 3 replicates/group). *P < 0.05, 1-way ANOVA and post hoc Tukey’s test.