Figure 1. CR3022 drives effector function against SARS-CoV-2.

(A) CR3022 was serially diluted and tested for its ability to bind the spike protein of different coronaviruses by ELISA (left). Data are represented as the OD₄₅₀ values background subtracted from the reference OD₅₇₀ value. Each dot represents the average of 2 replicates. The bar plot displays the EC₅₀ for each antigen. MERS RBD and Ebola virus (EBOV) glycoprotein are not displayed because their respective EC₅₀ was each infinite. The bars represent the average of 2 replicates, and the error bars represent the standard deviation. (B) CR3022 was serially diluted and preincubated with SARS-CoV-2 before adding to the virus and antibody to Vero E6 cells. Percentage neutralization was determined by the percentage reduction in plaque counts compared with a vehicle control. Data represent the means of triplicates and error bars represent the standard deviation. (C) CR3022 and a control EBOV-specific antibody binding to FcγR2a and 3a were evaluated using Luminex with a serial dilution of CR3022. The AUC was calculated for the MFI values. The bar represents the mean of 2 replicates. (D–G) CR3022 was evaluated for its ability to drive ADCP (D), NK activity (as measured by MIP-1b activity) (E), ADCD (F), and ADNP (G). For the line graphs (left), each dot represents the mean of 2 replicates. For the bar graphs, values are represented as the mean AUC of 8 serial dilutions run in replicate. The error bars for the dots and the AUC represent the standard deviation. For the before-after plot (E), each dot represents the activity of 1 donor after incubation with CR3022 or serum. Significance for NK activation (E) was determined by a Wilcoxon matched pairs signed rank test, *P < 0.05. (H and I) The ability of CR3022 and a control EBOV-specific antibody (KZ52) to drive ADCP (H) and ADCD (I) in the presence of ACE2 was analyzed. The bars indicate the average AUC of 8 serial dilutions run in 2 replicates. The error bars represent the standard deviation. Significance was determined by a 1-way ANOVA test followed by Tukey’s multiple-comparison test. *P < 0.05.