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. 2021 Jan 11;6(1):e141502. doi: 10.1172/jci.insight.141502

Figure 2. Anti-CD4 antibody clone SP35 specifically marks CAR+ cells.

Figure 2

(A and B) Specific CD4 (SP35) immunoreactivity in germinal centers from mesenteric lymph node sections from macaques that received either CD4CAR (A) or CD4CARΔζ (B); sparse marking in the parafollicular zone was also observed. (C and D) No immunoreactivity was seen in paired adjacent CD4CAR (C) or CD4CARΔζ (D) tissue sections labeled with an isotype control. (E) Positive control: Labeling of human tonsil shows specific immunoreactivity, which is predominately in the parafollicular zone and consistent with CD4+ T cell marking. (F) Negative control: no immunoreactivity is seen in a control mesenteric lymph node section from a macaque that did not receive either CD4CAR or CD4CARΔζ, indicating that the CD4 (SP35) antibody clone does not cross-react with the endogenous pigtail macaque CD4 antigen. Brown, immunoreactivity for human CD4CAR; blue, hematoxylin counterstain. The experiment was repeated twice to confirm the specificity of the CD4 (SP35) antibody for the human-derived CDCAR or CD4CARΔζ. Scale bars: 50 μm.