Skip to main content
. 2020 Dec 8;6(1):e139826. doi: 10.1172/jci.insight.139826

Figure 1. Adenine diet causes CKD with uremia.

Figure 1

(A) Experimental design for diet manipulation and timing of relevant outcome measures. (B) Quantification of body weights in control and CKD mice (n = 7–8/group/sex). (C) Quantification of kidney weights (n = 6–9/group/sex). (D) Glomerular filtration rate (GFR) measured by FITC-inulin clearance (n = 6–8/group/sex). (E) GFR results normalized to body weight (n = 6–7/group/sex). (F) Blood urea nitrogen (BUN) measured after 8 weeks on adenine diet and 10 weeks following 2 week washout before sacrifice (n = 6–8/group/sex). (G) Quantification of kidney fibrotic area (n = 3–6/group/sex). (H) Representative histological images (Masson’s trichrome) of control and CKD kidneys. (I) Serum metabolomics analysis for selected uremic toxins measured at sacrifice (n = 4/group/sex). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 using 2-way ANOVA and Tukey’s post hoc test when appropriate. Error bars show standard deviation. Scale bars: 200 μm.