Figure 5. CKD reduces activity of select matrix dehydrogenases but does not impair ETS enzyme activity.

Skeletal muscle mitochondria were isolated from control and CKD mice and subsequently permeabilized using alamethicin (0.03 mg/mL) or were lysed in cell lytic and underwent freeze thaw cycles for complex enzymatic assays (complexes I–V). (A) Matrix dehydrogenase activity was measured for the following enzymes: pyruvate dehydrogenase (PDH), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH), alpha ketoglutarate dehydrogenase (AKGDH), branched chain keto acid dehydrogenase (BCKDH), isocitrate dehydrogenase (ICDH), and malic enzyme (n = 5–7/group/sex). (B) Enzyme activity of the complexes of the ETS was assessed using standard biochemical assays (n = 6–7/group/sex). All data were analyzed using a 2-way ANOVA. Error bars show standard deviation.