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. 2020 Dec 8;6(1):e139826. doi: 10.1172/jci.insight.139826

Figure 9. Evidence of NMJ remodeling in CKD mice.

Figure 9

Representative immunofluorescence images of the NMJ morphology in TA muscle from control (A–D) and CKD mice (E–H). Images in panels C, D, G, and H are individual channels whereas A, B, E, and F are merged images. White arrows (E and F) demonstrate polyinnervation indicative of NMJ remodeling. NMJ labeling was performed using antibodies against the acetylcholine receptor (AChR), synaptophysin (terminal motor neuron), and neurofilament 200 (motor neuron axon). (I) Morphological analysis was performed by a blinded investigator for the following: number of nerve terminal branches and branch points, nerve terminal area, percentage area of AChR unoccupied, compactness of synaptophysin, and motor end plate area (n = 5 mice/group/sex; n > 3 NMJs/animal). All data analyzed using 2-way ANOVA and Tukey’s post hoc when appropriate. Error bars show standard deviation. Scale bars: 15 μm.