Figure 3.

Comparison of Leptospiral Immunoglobulin-Like B (LigB) Domain 12 and Invasin Domain 2 Structures. (A) The most stable NMR solution structures of LigB12 (PDB entry: 2MOG) from L. interrogans serovar Pomona type kennewicki strain JEN4 (26), and the D2 domain of invasion (PDB entry: 1CWV) from Yersinia pseudotuberculosis (27) are presented showing the immunoglobulin-like fold. LigB12 is composed of a β sandwich with ten β strands distributed over the two layers, with the more extensive divided into two smaller sheets. Invasin is composed of a β sandwich with ten β strands in two sheets. Note the strands extending from the amino- and carboxy-termini of the domain, which serve as linkages between domains. The images were obtained from the RCSB Protein Data Bank (28). (B) The secondary structure of LigB12 is represented as a topology diagram (26). Images from Ptak et al., 2014 (https://pubs.acs.org/doi/10.1021/bi500669u) (26), with permission from the American Chemical Society.