Table 6.
MACSPlex analysis of cytokine concentration in supernatants of the peripheral blood (PB) and native synovial fluid (SF)
| PB | SF | p values | |
|---|---|---|---|
| n | 10 | 10 | |
| GM-CSF | 0.61 (0.45–1.02) | 0.88 (0.40–2.04) | 0.655 |
| IFN-γ | 18.98 (10.53–33.80) | 15.49 (9.38–30.26) | 0.715 |
| IL-2 | 5.2 (2.74–7.69) | 5.51 (3.64–7.96) | 0.655 |
| IL-4 | 102.67 (67.65–146.47) | 87.37 (20.43–124.88) | 0.138 |
| IL-5 | 2.21 (1.21–4.16) | 2.81 (1.32–5.34) | 0.715 |
| IL-6 | 1.18 (0.62–1.84) | 19.45 (2.77–71.54) | 0.013* |
| IL-9 | 46.0 (40.72–54.29) | 40.81 (39.57–63.70) | 1.000 |
| IL-10 | 10.79 (8.86–19.85) | 8.12 (5.01–15.16) | 0.953 |
| IL-12 | 9.54 (6.05–21.58) | 6.96 (4.25–10.51) | 0.093 |
| IL-17A | 3.34 (1.79–4.02) | 3.84 (1.29–4.81) | 1.000 |
| TNF-α | 5.64 (4.43–7.72) | 7.73 (4.20–198.95) | 0.249 |
To evaluate multiple soluble immunomodulating cytokines in PB supernatants and native SF of early OA patients, we utilized the MACSPlex 12 Human Kit (Miltenyi, Germany). All work steps were performed according to the manufacturer’s instruction. Data is shown as median (IQR). Cytokine concentrations are given in pg/ml. Significant differences are marked with an asterisk: *p < 0.05