FIGURE 2.

Binimetinib decreases proliferation of AT/RT cells. (A) Photomicrographs of CHLA-06 and BT37 cells demonstrating smaller and fewer number of neurospheres with increasing binimetinib dose. (B) Immunofluorescence assay demonstrating decreased incorporation of BrdU with increasing concentration of binimetenib in CHLA-06 and BT37. On the left are representative images from one of 3 biological replicates. Cells stained with DAPI are in blue, while cells tagged with BrdU antibody are red. Calculated fraction of BrdU-positive cells in this particular biological replicate is shown on the right (*p < 0.05 by Student t-test, n = minimum of 3 technical replicates). (C) Western blot demonstrating decreasing S780 phospho-Rb expression with increasing concentrations of binimetinib compared with vehicle treated AT/RT cells. Relative phospho-RB expression levels as assessed by densitometry are shown above each image.