Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 20;41(3):538–554. doi: 10.1523/JNEUROSCI.0295-20.2020

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Fracassi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Figure 7. — A, A′, PPARα expression in frontal cortex of control, AD, and NDAN subjects. The quantitative analyses of the IF images showing upregulation of PPARα in AD compared with control subjects. NDAN and control subjects show comparable levels of the nuclear receptor. Magnification, 60×. Scale bar, 30 µm. Statistical analyses were made using one-way ANOVA (F_(2,15) = 52.78, p < 0.0001) following Tukey's test multiple-comparisons test. Values are expressed as the mean ± SD. ****p < 0.0001. B–D, PPARα expression and distribution in frontal cortex neurons and astrocytes of control, AD, and NDAN subjects. B, B′, Double IF of PPARα (green) in combination with NeuN (red) showing significant downregulation of the nuclear receptor in AD neurons. Magnification, 60×. Scale bar, 30 µm. Quantitative analysis of IF images showing a similar neuronal localization of PPARα in NDAN compared with AD subjects. Statistical analyses were made using one-way ANOVA (F_(2,15) = 31.94, p < 0.0001) following Tukey's test multiple-comparisons test. Values are expressed as the mean ± SD. ****p < 0.0001. C, C′, Double IF of PPARα (green) in combination with GFAP (red) and quantitative analysis showing a predominant localization of the nuclear receptor in AD astrocytes, while NDAN and control astrocytes display comparable levels of PPARα. Magnification, 60×. Scale bar, 30 µm. Statistical analyses were made using one-way ANOVA (F_(2,15) = 19.85, p < 0.0001) following Tukey's test multiple-comparisons test. Values are expressed as the mean ± SD. **p < 0.01; ****p < 0.0001. D, The analysis shows the significant upregulation of PPARα in AD astrocytes. NDAN and control subjects display comparable levels of PPARα in both neurons and astrocytes. Statistical analyses were made using two-way ANOVA (F_(2,30) = 42.54, p < 0.0001). Values are expressed as the mean ± SD. *p < 0.05; ***p < 0.001; ****p < 0.0001. ns, not significant.