Figure 9.

RFRP neuronal silencing does not prevent acute glucocorticoid-induced suppression of LH pulsatility in males. A, In response to 4 d of corticosterone treatment, male control mice and RFRP-hM4Di mice (n = 7 or 8 per group) both exhibited a significant suppression in average circulating LH concentration (p = 0.049). B, Similarly, male control and RFRP-hM4Di mice both exhibited a significant suppression in LH pulse frequency (p = 0.030). Data are mean ± SEM. Two-way ANOVA and Holm-Sidak's multiple comparisons tests were used for all analyses of LH data. C–F, Representative examples of LH profiles in control and RFRP-hM4Di male mice under basal conditions and in response to corticosterone treatment. Black circles represent pulse peaks. CNO (2 mg/kg s.c.) was injected 30 min before the beginning of LH pulse sampling.