Skip to main content
. 2020 Dec 4;6(49):eabc6983. doi: 10.1126/sciadv.abc6983

Fig. 2. Differentiation of MS-hiOLs into mature oligodendrocytes is timely regulated in the corpus callosum of the developing Shi/Shi:Rag2−/− brain.

Fig. 2

(A) Combined immunodetection of human nuclei marker STEM101 (red) with CC1 (green) and SOX10 (white) for control (top) and MS-hiOLs (bottom) at 8, 12, and 16 wpg. (B and C) Quantification of SOX10+/STEM+ cells (B) and CC1+ SOX10+ over STEM+ cells (C). While the percentage of human oligodendroglial cells increased only slightly with time, the percentage of mature oligodendrocytes was significantly time regulated for both MS- and control-hiOLs. Two-way ANOVA followed by Tukey’s multiple comparison tests were used for the statistical analysis of these experiments (n = 3 to 4 mice per group). Error bars represent SEMs. *P < 0.05 and ****P < 0.0001. Scale bar, 100 μm.