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. 2020 Dec 9;6(50):eabb1654. doi: 10.1126/sciadv.abb1654

Fig. 3. cf-mRNA reveals gene signatures that correlate with AD severity and patient heterogeneity of cognitive impairment in patients with AD.

Fig. 3

(A) Consensus NMF matrix. Unsupervised NMF clustering from 2591 differentially expressed genes. Six gene cluster subtypes are highlighted in color. (B) Top three most significant pathways (IPA) identified using gene set enrichment analysis for each cluster. The black horizontal dotted line represents significance threshold (P < 0.05). GTPases, guanosine triphosphatases. (C) Unsupervised clustering of patients with AD using their cf-mRNA profile based on NMF clusters identified in (A) revealed five groups of patients. (D) The expression of synaptic transmission and immune and inflammatory response clusters categorized by CDR rating. (E) Overlapping features between genes that correlate with Mini Mental State Exam (MMSE) and CDR scores. Top 20 genes that most highly correlated with MMSE and CDR scores are shown in the rectangular box. (F) The expression of PROK2 as a function of CDR (left) and MMSE (right) scores.