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. 2020 Dec 4;6(49):eabd0130. doi: 10.1126/sciadv.abd0130

Fig. 4. ML355-sHDL efficiently inhibits thrombus formation in laser-induced cremaster arteriole thrombosis models.

Fig. 4

Male mice (n = 4) were pretreated intravenously with saline control, sHDL (50 mg/kg), ML355 (1.5 mg/kg), or ML355-sHDL (sHDL at 50 mg/kg and ML355 at 1.5 mg/kg). After 24 hours, mice were anesthetized and surgically prepared as described in detail in Materials and Methods. Multiple independent thrombi were induced and recorded as described above. (A) Representative captured thrombi from each group at different time points. (B) Dynamics of platelet accumulation in thrombi assessed by relative mean fluorescence intensity of platelet averaged by 8 to 10 thrombi per mouse, three mice in each group. All thrombi were statistically analyzed for the change of fluorescence intensity over the course of thrombus formation after subtracting fluorescent background defined on an uninjured section of the vessel using the SlideBook program. Data are means ± SD and compared by two-way analysis of variance (ANOVA) analysis for statistical difference. ***P < 0.001.