Fig. 5. ML355-sHDL efficiently delays vessel occlusion in FeCl₃-induced carotid artery thrombosis model.

Both female and male mice (n = 6) were pretreated intravenously with saline control, sHDL (50 mg/kg), ML355 (1.5 mg/kg), or ML355-sHDL (sHDL at 50 mg/kg and ML355 at 1.5 mg/kg). After 24 hours, mice were anesthetized and surgically prepared as described in detail in Materials and Methods. (A) Representative images of thrombosis in carotid artery in response to FeCl₃ injury. FeCl₃ (10%) was topically applied on the right carotid artery for 3 min, images of thrombi were recorded in real time, and vessel occlusion was determined by the secession of blood flow. (B) Carotid artery vessel occlusion time in each group. The mean vessel occlusion time in the control mice, ML355, sHDL, and ML355-sHDL was 7.1 ± 1.2 min, 11.0 ± 2.3 min, 11.3 ± 1.3 min, and 21.4 ± 6.4 min, respectively. Data are means ± SD (n = 6). **P < 0.01, ***P < 0.001.