Table 2.
Enzyme inhibitory and antiproliferative activities of the derived hydrazide compounds. 
| Compounds | R1 | R2 | HDACsa | U266b |
|---|---|---|---|---|
| I1e1 | –H | –C2H5 | 50.60 ± 2.02 | 15.97 ± 0.14 |
| I1e2 | –H | –(CH2)2CH3 | 61.34 ± 3.56 | 0.64 ± 0.02 |
| I1e3 | –H | –(CH2)3CH3 | 30.47 ± 1.17 | 1.06 ± 0.05 |
| I1e4 | –H | –CH2CH (CH3)2 | 7.16 ± 1.50 | 15.98 ± 0.67 |
| I2e2 |
|
–(CH2)2CH3 | 57.46 ± 2.06 | 1.11 ± 0.08 |
| I2e3 |
|
–(CH2)3CH3 | 51.41 ± 2.78 | 1.71 ± 0.07 |
| I3e2 | –C2H5 | –(CH2)2CH3 | 36.51 ± 2.93 | 0.48 ± 0.10 |
| I3e3 | –C2H5 | –(CH2)3CH3 | 31.94 ± 1.51 | 1.45 ± 0.10 |
| I7e2 |
|
–(CH2)2CH3 | 30.45 ± 3.38 | 1.32 ± 0.08 |
| I10e2 |
|
–(CH2)2CH3 | 46.85 ± 2.06 | 0.74 ± 0.05 |
| I12e2 | –(CH2)3CH3 | –(CH2)2CH3 | 39.73 ± 1.84 | 1.28 ± 0.12 |
| I12e3 | –(CH2)3CH3 | –(CH2)3CH3 | 42.76 ± 1.56 | 1.44 ± 0.31 |
| I13e2 | –CH3 | –(CH2)2CH3 | 29.68 ± 2.06 | 0.71 ± 0.11 |
| I13e3 | –CH3 | –(CH2)3CH3 | 32.15 ± 1.03 | 1.36 ± 0.06 |
| SAHA | 59.91 ± 3.73 | 0.88 ± 0.08 |
a
Percentage inhibition rate (%, at the concentration of 1 µM), HeLa nucleus extract was used for the test. Each value is the mean of at least three experiments.
b
IC50, µM. Each value is the mean of at least three experiments.